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Introduction
The development of physical culture, formation of a healthy lifestyle, and assessment of physical working capacity represent a комплекс of preventive measures aimed at preventing diseases, strengthening all body systems, and improving a person’s overall well-being [1–3; 9–14].
A healthy lifestyle is an individual system of behavior, and the choice of this positive life path must ultimately be made by each person independently. Helping children understand this responsibility represents an important direction in health education and a significant potential that remains insufficiently utilized. Children need knowledge about health and a healthy lifestyle, and it is essential to provide them with this knowledge and teach them how to apply it in everyday life.
It is necessary to teach children to formulate clear life goals and develop psychological resilience in various life situations. They should understand behavioral patterns that contribute to maintaining and strengthening their health.
Combat Horting coaches should cultivate in boys and girls a positive attitude toward life, teach them to take responsibility for their own lives, and develop a sense of self-respect and personal dignity [15–18]. All these components are integral elements of a healthy lifestyle. Once children become aware of the importance of these principles and understand the correctness of this life approach, they will be able to follow healthy lifestyle guidelines more easily, relying on the knowledge and skills they have acquired.
Purpose of the Study
The purpose of this scientific research is to determine the main parameters of the methodology for developing physical culture, forming a healthy lifestyle, and assessing the physical working capacity of school and university student athletes engaged in Combat Horting. The study also aims to develop a conscious and responsible attitude among school and university students toward their own health and the health of the Ukrainian nation.
The Influence of Combat Horting as a Sport on Human Health
Many people strive to achieve strong health and a physically fit body; however, the path toward these goals is often chosen incorrectly. Some people postpone physical activity until “Monday,” begin training for several weeks, and then abandon sport due to a loss of motivation. Others spend years searching for a “magic solution,” believing advertisements promoting ineffective products.
However, there is only one scientifically proven approach that, when applied rationally, does not create additional health risks but, on the contrary, strengthens the body and allows a person to achieve optimal physical condition. Regular Combat Horting training combined with balanced nutrition and an appropriate caloric balance, depending on individual goals, represents a lifestyle choice that can positively transform a person’s physical and psychological well-being.
The positive impact of physical activity on human health is undeniable, as demonstrated by thousands of athletes and hundreds of scientific studies. Nevertheless, some individuals believe that success in Combat Horting training depends primarily on genetic factors and that the health benefits of intensive sports participation are questionable. They often refer to examples of former elite athletes who have experienced health problems after leaving professional sports.
At the same time, medical science has thoroughly studied the effects of physical activity on human health. For example, therapeutic physical education methods are widely and successfully used for the rehabilitation of various population groups — from recovery after major surgeries and medical procedures to improving the health status of older adults with chronic diseases.
Currently, the National Federation of Combat Horting of Ukraine conducts comprehensive research aimed at determining the influence of Combat Horting training on the functional condition of various body systems.
Influence of Physical Activity on the Cardiovascular System
Any type of physical activity affects the cardiovascular system. This influence can be both positive and negative. When analyzing the impact of sports on heart function, it is important to understand that negative consequences may occur when an untrained person suddenly begins excessively intensive training and physical loads exceed the adaptive capabilities of the body.
As a result, health problems such as cardiac arrhythmia, cardiomyopathy, and hypotension may occur. In contrast, moderate and properly structured training improves cardiovascular function and contributes to better adaptation of the body to physical stress.
General physical aerobic activities (walking, running, cycling, swimming, and similar exercises) are often referred to as cardiovascular training because they primarily stimulate the heart and circulatory system. Their popularity is partly explained by the fact that the intensity of such activities can be easily controlled through movement speed, duration, and training frequency.
Individuals who regularly participate in aerobic training can observe that, over time, the body adapts to physical loads and becomes increasingly capable of performing cardiovascular exercises more efficiently.
Therefore, it is important to select appropriate physical activities and determine their optimal intensity. If a person has chronic diseases or other health-related problems, it is essential to consult a physician beforehand. During the initial stages of Combat Horting training, participation under the supervision of an experienced instructor is recommended. It is also important to maintain an active lifestyle during leisure time — spend more time walking outdoors in summer, engage in skiing or skating during winter, and participate in hiking activities [22].
A significant contribution to the development of the problem of physical culture education and health fundamentals among schoolchildren and students has been made by the scientific works of I. D. Bekh, V. V. Vekhtev, Z. M. Dikhtiarenko, N. Yu. Dovhan, S. V. Yeresova, S. I. Zhevaha, M. D. Zubalii, V. V. Ivashkovskyi, S. A. Karasevych, R. V. Kiblitskyi, O. I. Ostapenko, M. V. Tymchyk, and other researchers [4; 7; 8].
Currently, researchers of the National Federation of Combat Horting of Ukraine have intensified their attention toward studying the formation of a healthy lifestyle among school and university students through the implementation of health-improving and training methods based on Combat Horting activities and health-preserving technologies. These approaches represent one of the most effective methods for developing positive behavioral characteristics associated with a healthy lifestyle.
By highlighting health-oriented methodologies of Combat Horting, contemporary research contributes to the formation of a scientific worldview, a methodologically correct understanding of physical culture and health fundamentals, and awareness of the relationship between this field and the concept of a healthy lifestyle not only among children and adolescents but also among their parents [20].
Presentation of the Main Research Content and Results
Every person strives to achieve as much as possible in life and accomplish personal goals; however, not everyone succeeds in achieving these objectives. The pace of modern life continues to accelerate, while human resources and energy levels do not always increase accordingly.
Although people today generally live in much greater material comfort than their ancestors did a century ago, this does not necessarily mean that they feel happier or healthier. Improvements in living standards have also brought new challenges, including an increase in overweight and obesity, a growing prevalence of cardiovascular diseases, psychological difficulties, and other health-related issues.
How can these problems be addressed? According to many researchers, one of the main causes is the insufficient ability of individuals to manage modern benefits and opportunities effectively. Reduced self-discipline, weakened willpower, and unwillingness to make efforts to achieve personal goals negatively influence human health. One effective way to overcome these unfavorable factors is participation in sports activities and maintaining an active lifestyle.
Physical activity is a fundamental function of a living organism. Many modern people believe that the main characteristics of human life are social success, professional achievements, communication skills, spiritual values, and intellectual development. At the same time, they often underestimate the importance of regular physical activity.
However, the foundation of human existence is biological in nature and is based on anatomy and physiology. Although intellectual activity cannot be reduced solely to neurophysiological processes, it is impossible to deny that healthy physical development of the human body is a necessary foundation for psychological and spiritual activity.
In this context, an important principle established by biologists and medical researchers long ago should be emphasized: an organ whose function is not sufficiently utilized gradually loses its effectiveness, undergoes degradation, and its tissues may atrophy.
If a person does not regularly engage muscles in physical activity, they do not develop but instead weaken and lose functional capacity. However, muscle deterioration is only one aspect of the problem. More serious consequences include impaired blood circulation in muscle tissues, increased accumulation of adipose tissue, and deterioration of neuromuscular coordination.
Thus, muscle atrophy is accompanied by negative changes in the nervous system, cardiovascular system, and musculoskeletal system. The brain, receiving less oxygen and fewer stimulating nerve impulses from muscles, also begins to function less effectively. As a result, the body's ability to resist aging processes and certain diseases, including neurodegenerative disorders such as Alzheimer’s disease, may decrease.
Therefore, an inevitable conclusion follows: in order to maintain health and functional longevity, the human body must remain physically active. From a biological perspective, humans are naturally designed to perform movements such as running, jumping, hanging, pulling themselves upward, bending, lifting objects, squatting, and performing push-ups. These basic biological movements ensure the normal functioning of the body.
When these functions are not realized, the organism becomes weaker, loses adaptive capabilities, and becomes more vulnerable to various diseases.
Physical activity for humans is not a luxury but an essential condition for maintaining health and survival. In this context, sport should not be understood only as an entertainment industry or as professional competition aimed at achieving exceptional results. Rather, it should be considered as an element of physical culture and a component of a healthy lifestyle.
Many traditional and modern forms of physical activity practiced at the amateur level are capable of providing Combat Horting athletes with effective physiological recovery after training loads, maintaining functional balance, and supporting a healthy lifestyle.
Benefits of Combat Horting Training for Physical, Psychological, and Social Development
1. Sports games, physical activities, and systematic training maintain normal body functionality and contribute to harmonious physical development. They ensure the full functioning of all body systems and organs, prevent blood circulation stagnation in specific areas of the body, reduce excessive fat accumulation, and help avoid excessive stress on joints, ligaments, and the cardiovascular system. Regular physical activity improves neuromuscular coordination and allows physiological systems to function more efficiently, activating not only their direct functions but also recovery processes, regeneration, and the formation of new tissues to replace damaged structures.
2. Systematic participation in sports contributes to a richer emotional life. It provides opportunities to release negative emotions and experience a wide range of psychological states — from the disappointment of defeat, the joy of communication, and the feeling of relaxation after intensive training to the anticipation of a Combat Horting match and the emotional satisfaction of victory.
In many cases, sport allows individuals to experience emotions and psychological states (such as triumph, achievement, and collective success) that may be unavailable in everyday life due to various social and psychological factors.
3. Regular training strengthens character, develops willpower, promotes discipline, and teaches individuals to achieve success through gradual and consistent efforts. Team competitions in Combat Horting provide valuable experience in cooperation, leadership, responsibility, overcoming physical discomfort, and making maximum effort for collective achievement.
The achievement of personal goals — whether improving physical appearance, increasing strength indicators, performing a greater number of pull-ups, mastering technical Combat Horting techniques, or successfully executing throws and competitive actions against an opponent — allows individuals to experience confidence in their abilities, overcome feelings of failure, and discover their potential for personal growth and self-development.
All these advantages provided by Combat Horting training are available to people of different ages. The key factor is the correct selection of training modes, physical loads, and recovery periods. For children and adolescents, this sport is especially valuable because it promotes physical development, social adaptation, discipline, responsibility, and personal growth.
During the training process, Combat Horting coaches, together with sports physicians and specialists in sports medicine, analyze athletes’ functional capacity and physical working ability using the PWC170 test.
Assessment of Physical Working Capacity of Combat Horting Athletes of Different Qualification Levels Using the PWC170 Test
The functional test based on determining the level of muscular workload at which heart rate increases to 170 beats per minute is known as the Sjöstrand test or the PWC170 test (derived from the English term Physical Working Capacity).
Assessment of physical working capacity using the PWC170 test is based on two established physiological principles of muscular activity:
- the increase in heart rate during muscular work is directly proportional to the intensity (power) of the performed activity;
- the degree of heart rate increase during a given physical workload is inversely proportional to an individual’s ability to perform muscular work of that intensity, which reflects their physical working capacity.
Therefore, heart rate response during muscular activity can be considered a reliable criterion for assessing the physical working capacity of Combat Horting athletes.
There are two main approaches to determining physical working capacity based on heart rate response to physical exercise:
- assessment of heart rate during the performance of standardized muscular work;
- determination of the workload intensity at which heart rate increases to a standardized level.
The second approach is considered more scientifically justified because it forms the basis of the PWC170 test methodology.
The selection of a heart rate level of 170 beats per minute is based on the fact that at this intensity the cardiovascular system continues to function within an optimal physiological range. In other words, at heart rates up to 170 beats per minute, there is a direct linear relationship between the power of muscular work and heart rate, while ATP (adenosine triphosphate) production occurs primarily through aerobic oxidation processes.
At higher heart rates, this linear relationship becomes disrupted due to increased activation of anaerobic (glycolytic) energy supply mechanisms during muscular activity.
Methods of Conducting the PWC170 Test
In sports practice, two main variations of the PWC170 test are commonly used:
- step test, in which physical workload is performed through repeated stepping onto a platform of a standardized height;
- cycle ergometry, in which workload is performed using a stationary bicycle ergometer.
Methodology for Determining PWC170 Using the Step Test
The height of the step most commonly used in the test is equal to approximately half of the length of the participant’s leg. Therefore, it is recommended to have a set of platforms with different heights (15, 30, 40 cm, etc.) to select an appropriate workload for each athlete.
The power of the performed physical work (W) can be determined using the following formula or by using standardized reference tables:
W(kgm/min)=1.33×p×h×n
where:
- 1.33 — coefficient accounting for the amount of work performed during the downward movement from the step;
- p — body mass of the participant (kg);
- h — height of the step (m);
- n — number of step cycles per minute.
For example, assume that the participant’s body mass is 75 kg and the step height is 40 cm. The required stepping rate to achieve a workload of 1000 kgm/min can be calculated as follows:
1000kgm/min=1.33×0.4×75×X X=1.33×0.4×751000=25 cycles/min
Since one step cycle consists of four individual steps, 25 cycles correspond to a metronome tempo of 100 beats per minute.
The obtained value of physical working capacity of a Combat Horting athlete expressed in kgm/min can be converted into watts (W) by dividing the result by 6, because:
Procedure for Conducting the PWC170 Step Test
After selecting the optimal step height, the test is performed according to the following sequence:
1. Using Table 1, determine the individual number of step elevations according to the selected step height, the participant’s body mass, and the planned power of the first workload.
2. Apply electrocardiograph electrodes to record heart rate at the end of each workload stage (based on the R–R intervals of the electrocardiogram, ECG).
3. Perform the first workload (W₁) at a stepping rate of one cycle every two seconds for 5 minutes. Record the ECG during the final 30 seconds of the fifth minute to determine heart rate (f₁).
4. Rest in a sitting position for 3 minutes.
5. Perform the second workload (W₂) for 5 minutes. The number of step elevations and the step height are determined according to Table 2. The stepping tempo remains the same. ECG recording is performed using the same procedure as after the first workload.
6. Determine the PWC170 value using either a graphical or mathematical calculation method.
Table 1
Determination of the Performed Workload Power (kgm/min) During the Step Test Depending on the Body Mass of Combat Horting Athletes, Step Height, and Number of Step Cycles
(Number of step cycles per minute)
Body mass, kg
| Step height |
Step cycles/min |
40 |
45 |
50 |
55 |
60 |
65 |
70 |
75 |
80 |
85 |
90 |
95 |
100 |
| 15 cm |
10 |
80 |
90 |
100 |
110 |
120 |
129 |
140 |
150 |
160 |
169 |
180 |
190 |
200 |
| |
15 |
120 |
135 |
150 |
165 |
180 |
195 |
210 |
225 |
239 |
254 |
270 |
284 |
299 |
| |
20 |
160 |
179 |
200 |
219 |
239 |
260 |
279 |
300 |
319 |
340 |
359 |
379 |
399 |
| |
25 |
200 |
224 |
249 |
274 |
300 |
324 |
349 |
374 |
399 |
424 |
449 |
474 |
499 |
| |
30 |
239 |
270 |
300 |
330 |
360 |
390 |
420 |
450 |
479 |
509 |
539 |
568 |
598 |
| |
35 |
279 |
314 |
349 |
384 |
419 |
454 |
488 |
524 |
559 |
593 |
629 |
663 |
698 |
| |
40 |
319 |
358 |
399 |
439 |
479 |
519 |
559 |
598 |
638 |
696 |
718 |
758 |
798 |
(The complete table should continue with 30 cm and 40 cm step heights in the same format.)
Graphical Method for Determining PWC170
The graphical determination of PWC170 is performed as follows: the power values of two consecutively performed workloads are plotted on the x-axis (abscissa), while the corresponding heart rate values are plotted on the y-axis (ordinate).
Two points are obtained based on the relationship between workload power and heart rate response. A straight line is drawn through these two points until it intersects the heart rate level of 170 beats per minute (bpm).
From the obtained intersection point, a perpendicular line is projected onto the x-axis. The resulting value represents the workload power corresponding to a heart rate of 170 bpm, which is defined as the athlete’s PWC170 value (Figure 1).
Figure 1. Graphical method for determining PWC170
where:
- HR₁ and HR₂ — heart rate values recorded after the first and second workloads;
- W₁ and W₂ — power values of the first and second workloads.
Methodology for Determining PWC170 Using a Cycle Ergometer
The participant is instructed to perform two consecutive workloads of moderate intensity (for example, 500 and 1000 kgm/min) with a pedal cadence of 60–80 revolutions per minute, separated by a three-minute rest interval.
Each workload lasts 5 minutes. At the end of each workload stage, heart rate (HR) is determined during the final 30 seconds using the auscultatory method (stethophonendoscope) or recorded by electrocardiography (ECG) for the same purpose.
The most rational approach for calculating PWC170 is not the graphical method but the mathematical method based on the substitution of heart rate and workload power values into the formula proposed by V. L. Karpman et al. (1974):
PWC170=W1+(W2−W1)f2−f1170−f1
where:
- PWC170 — physical working capacity corresponding to a heart rate of 170 beats per minute (kgm/min);
- W₁ — power output of the first workload (kgm/min);
- W₂ — power output of the second workload (kgm/min);
- f₁ — heart rate at the end of the first workload (beats/min);
- f₂ — heart rate at the end of the second workload (beats/min).
This equation allows the calculation of PWC170 when the power values of the first and second workloads and the corresponding heart rate responses are known.
Conditions for Reliable Determination of PWC170 in Combat Horting Athletes
The assessment of physical working capacity of Combat Horting athletes through PWC170 calculation provides reliable results only when certain methodological conditions are met.
When the difference between the first and second workloads is insufficient, the accuracy of PWC170 determination decreases. This occurs mainly because the cardiovascular regulatory system cannot accurately differentiate between workloads with very similar power values.
Therefore, during PWC170 testing, the power output of the second workload should differ substantially from the first workload. Recommended workload values that ensure reliable PWC170 determination are presented in Table 2.
Using these reference values, it is relatively easy to select appropriate workload intensities. The correctness of workload selection can be evaluated by the heart rate response at the end of each stage.
The expected physiological response should be:
- after the first workload: heart rate should reach approximately 100–120 beats/min;
- after the second workload: heart rate should reach approximately 160–170 beats/min.
The PWC170 test demonstrates sufficient methodological validity and reliability. An important advantage of the PWC170 procedure is that during testing it is possible to observe the participant’s subjective response and perception of the physical workload.
Table 2
Recommended Power Output of the First Workload (W₁, kgm/min) for Determining PWC170 in Combat Horting Athletes of Different Body Mass and Physical Ability Profiles
| Physical abilities developed in different Combat Horting disciplines |
Body mass, kg |
50–59 |
60–64 |
65–69 |
70–74 |
75–79 |
80–84 |
>85 |
| Speed-strength and complex coordination abilities |
|
400 |
400 |
500 |
500 |
500 |
600 |
600 |
| Competition section “Hand-to-hand Combat” |
|
400 |
400 |
500 |
600 |
700 |
800 |
800 |
| Endurance-oriented disciplines |
|
540 |
600 |
700 |
800 |
900 |
900 |
1000 |
Among Combat Horting athletes, approximately 8.2% of individuals have a body mass below 55 kg, and nearly 91.0% of this group are female athletes.
Based on empirical data, recommendations have been developed for selecting the first workload power during cycle ergometer testing of Combat Horting athletes with body mass below 55 kg.
It is proposed to expand the lowest body mass category and begin classification from 50 kg. The recommended power output of the first workload should be established as follows:
- for speed-strength, complex coordination, game sports, and martial arts disciplines — 400 kgm/min;
- for endurance-oriented sports disciplines — 540 kgm/min.
Adjustment of PWC170 Testing Parameters in Combat Horting Athletes with Resting Bradycardia
It has been observed that among highly qualified Combat Horting athletes, 28.6% demonstrate resting heart rates within the range of 48–60 beats/min. Among these athletes, 65.6% are males and 34.4% are females.
During the submaximal PWC170 test, athletes with resting bradycardia demonstrate a heart rate response after the first cycle ergometer workload ranging from 85–90 beats/min to 100–120 beats/min. This physiological response may lead to an unjustified increase in the power of the second workload, resulting in a heart rate exceeding 170 beats/min at the end of the test and causing inaccuracies in the calculation of physical working capacity.
To prevent such errors, it is recommended to increase the power output of the first cycle ergometer workload:
- by 120–180 kgm/min when resting heart rate is within 55–60 beats/min;
- by 180–240 kgm/min when resting heart rate is within 48–54 beats/min.
The recommended values for calculating the second workload power required for reliable determination of PWC170 are presented in Table 3.
Table 3
Recommended Power Output of the Second Workload (W₂) for Determining PWC170
| Power output of the first workload (W₁), kgm/min |
Heart rate during W₁ (beats/min) |
90–99 |
100–109 |
110–119 |
120–129 |
| 300 |
|
1000 |
850 |
700 |
600 |
| 400 |
|
1200 |
1000 |
800 |
700 |
| 500 |
|
1400 |
1200 |
1000 |
850 |
| 600 |
|
1600 |
1400 |
1200 |
1000 |
| 700 |
|
1800 |
1600 |
1400 |
1200 |
| 800 |
|
1900 |
1700 |
1500 |
1300 |
| 900 |
|
2000 |
1800 |
1600 |
1400 |
Evaluation and Interpretation of Physical Working Capacity Results
The accumulated scientific data concerning the assessment of physical working capacity among athletes of different sports specializations require further analysis and interpretation, as sports physicians and coaches often demonstrate different approaches to evaluating the obtained results.
Many coaches, particularly those working with athletes involved in cyclic endurance-oriented sports, attempt to improve the physical working capacity of their athletes using all available methods. In many cases, such efforts lead to increased indicators of physical working capacity but do not necessarily result in competitive success [23].
This phenomenon is associated with the fact that excessive focus on improving endurance characteristics may negatively affect athletes’ speed abilities, which often determine success in high-level competitions.
Physical working capacity is evaluated by analyzing individual changes in PWC170 over time and comparing these values with established reference standards for specific population groups. It is evident that the higher the PWC170 value, the greater the amount of mechanical work that a Combat Horting athlete can perform while maintaining optimal cardiovascular system functioning.
Therefore, higher PWC170 values indicate a higher level of physical working capacity.
The level of physical working capacity determined by the PWC170 test primarily reflects the functional efficiency of the cardiorespiratory system. The more effectively the cardiovascular system functions, the greater the adaptive capabilities of the body's regulatory mechanisms and the higher the PWC170 value.
Significant influence on PWC170 values is also exerted by physical development characteristics. Absolute PWC170 values are directly dependent on body size and body mass. Therefore, to eliminate individual differences associated with body mass, relative PWC170 values are calculated per kilogram of body weight.
Further interpretation of the obtained results is based on relative indicators:
Individual variations in PWC170 values are also influenced by sex, age, genetic factors, health status, and the level of physical activity.
In healthy young men who do not participate in sports (mean age 23.9 ± 6.1 years), the average PWC170 values are:
- 1001 ± 136 kgm/min;
- PWC170/kg — 14.4 ± 2.7 kgm/min/kg.
In women (mean age 24.1 ± 2.6 years), these values are:
- 640 ± 105 kgm/min;
- PWC170/kg — 10.2 ± 1.6 kgm/min/kg.
These data represent statistical indicators of physical working capacity among non-athletic individuals according to V. L. Karpman et al. (1988) and are presented as mean values (X ± δ).
Possible Exercise-Induced Oxidative Stress Associated with Combat Horting Physical Training
Terms such as free radicals, oxidative stress, and antioxidants, which are frequently used in sports advertising to attract the attention of athletes and coaches, refer to complex molecular structures, physiological processes, and biochemical substances.
During normal metabolism, oxygen is utilized by mitochondria for energy production. A small percentage of oxygen is not completely reduced, resulting in the formation of intermediate products known as reactive oxygen species (ROS).
Some of these substances are classified as free radicals, a term widely used in antioxidant-related discussions.
Reactive oxygen species can damage important cellular structures, including cell membranes, leading to impairment of cellular functions and physiological processes.
During Combat Horting physical exercises, oxygen consumption increases significantly compared with resting conditions. During intensive muscular activity, oxygen consumption in mitochondria of actively contracting muscle cells may increase many times above resting levels. As oxygen consumption increases, mitochondrial production of ROS also rises.
The discovery of this mechanism has led to scientific interest regarding the potential negative effects of increased ROS production on the health of Combat Horting athletes [19].
However, the human body possesses a complex and highly effective antioxidant defense system designed to protect cells from excessive ROS activity.
This defense system includes:
- enzymatic antioxidants;
- non-enzymatic antioxidants;
- dietary antioxidants.
Examples include:
- glutathione (non-enzymatic antioxidant);
- superoxide dismutase (enzymatic antioxidant);
- vitamin E (dietary antioxidant).
The protective action of antioxidants occurs through neutralization of ROS molecules or elimination of intermediate products involved in ROS formation.
During Combat Horting training, when ROS levels increase significantly, there is a possibility that antioxidant defense mechanisms may become insufficient. This unstable physiological condition is known as exercise-induced oxidative stress.
The primary biological targets of oxidative stress include:
- proteins;
- lipids;
- DNA molecules.
Oxidative stress is associated with numerous pathological conditions, including cardiovascular diseases, inflammatory disorders, metabolic disorders, neurodegenerative diseases, cancer development, and biological aging processes. This area of research continues to expand.
Nevertheless, although ROS are associated with potentially harmful biological processes, they are also essential for normal cellular development and optimal physiological functioning.
Reactive oxygen species perform important signaling functions, participate in gene activation, regulate cellular growth, influence biochemical reactions, and contribute to the biosynthesis of various molecules.
Modern research conducted over several decades has demonstrated that maintaining an appropriate oxidant–antioxidant balance is essential for cellular survival and normal physiological function.
Therefore, attempts to artificially disrupt this balance — for example, through excessive use of antioxidant supplements — may potentially lead to negative consequences for athlete health, including Combat Horting athletes.
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